IN VITRO CLONAL PROPAGATION OF VULNERABLE MEDICINAL PLANT PHYSALIS MINIMA L.

Author(s): Karpaga Sundari. C, Ramesh. S and Chandran.C

Abstract: In vitro method for mass propagation of valuable medicinal plant Physalis minima L. belongs to the family Solanaceae, direct from shoot tip, nodel and leaf explants. In the present study direct organogenesis shoot multiplication in to nodal explants was higher than shoot tip explants and maximum percentage of response and number of shoots were noticed on the medium containing 2.0 mg/l of BAP and KN. Leaf explants failed to produce multiple shoot even on repeated subcultures. Callus induction and organogenesis of three different explants (shoot tip, node and leaf) showed positive response for callus induction on MS medium containing auxin (NAA) and cytokinins (BAP & KN). The regenerated shoots from all the explants were responded well for rooting on MS medium supplemented with IBA or IAA (2.0 mg/l). During the process of hardening, the rooted plantlets were transferred to the sterilized mixture of garden soil, farmyard manure, and sand (2:1:1) showed 80% survival and were identical to the donar plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variations.

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