CHARACTERIZATION OF MICROBIALLY SYNTHESIZED SILVER NANOPARTICLES AND ITS APPLICATION IN DYE DECOLORIZATION

Author(s): Nayan Jani, Nafisa Patel*and Mithil Parekh

Abstract: Biological approach for synthesis of nanoparticles using microorganisms received great interest because of their potential to synthesize nanoparticles of different sizes, shape, and morphology. Microbial synthesis is known as green synthesis because neither hazardous chemical are required for the synthesis nor any hazardous by product is generated. Silver nanoparticles gain more attention because of their wide application is various fields like medical, agriculture, bio-remediation and industrial. The present study is focused on synthesis of silver nanoparticles by microorganism isolated from stressed environment of industrial effluents. Among 7 isolates 4 isolates were screened positive for synthesis of nanoparticles. Color changes from pale yellow to brown due to reduction of silver nitrate with culture supernatant indicates formation of silver nanoparticles. Characterization of synthesized nanoparticles were carried out by UV-visible spectrophotometer which showed silver surface plasmon vibration band near 420nm. Average size of synthesized particles ranged from134-207 nm. FTIR (Fourier transform infrared spectroscopy) analysis showed 3 major peaks at 3433.90 cm, 2062.28 cm, 1633.64 cm indicative of the synthesized particles with the bacterial isolates. Photo catalytic degradation of dyes viz. methylene blue and brilliant red was studied using the microbially synthesized nanoparticles. Methylene blue decolorization was observed to be 64% and 49% decolorization of brilliant red within 24 hours of exposure. These microbially synthesized silver nanoparticles could be an effective alternative for the treatment of textile effluent.

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