SCREENING AND CHARACTERIZATION OF PROTEASE PRODUCING HALOPHILIC BACTERIA FROM SALTPAN AREA VEDARANYAM, TAMIL NADU

Author(s): Manikandan P, Gnanasekaran A and Senthilkumar P.K*

Abstract: This study was focused on screening and characterization of protease producing bacterial strain from saltpan located in vedaranyam. The isolated potential bacterial strain was identified as Bacillus firmus by various staining, biochemical tests includes 16S rRNA sequencing method. The effects of various environmental factors on the production of protease were studied. The organism showed the significant growth and enzyme production at pH 9.0 and temperature range at 37ºC. The protease enzyme was analyzed and purified by using ammonium sulphate precipitation method and membrane dialysis. The protease enzyme liberated from these strains showed better stability and activity in pH 8.0 and at temperature 40˚C suggesting that enzyme used in antibacterial activity and pharmaceutical applications.

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