ETHANOL EXTRACT OF Passiflora edulis f. Flavicarpa Degener LEAVES SUPPRESSES ADIPOGENESIS BY DOWN REGULATION OF PPAR -γ IN IN VIVO AND EX VIVO MODELS

Author(s): Jinitha Anu Saji and Veena R*

Abstract: The anti-adipogenic as well as antihyperlipidemic effects in vivo of the ethanolic extract of Passiflora edulis f. Flavicarpa Degener leaves (EEPE) were investigated. The pharmacological effects were assessed by measuring bodyweight (every 5th day), serum total lipid profile (30th day) using High fat diet (HFD) induced model. After cervical dislocation, the in vivo antioxidant property was analysed by lipid peroxidation assay using rat liver slices. The liver slices were also subjected to reverse transcriptase PCR to analyse expression of PPAR-γ gene in obesity and adipogenesis. Significant decrease in weight was observed along with marked decrease in lipid profile levels as well as atherogenic index too, demonstrated the antihyperlipidemic activity of the extract. The percentage of lipid peroxidation of extract treated was substantially reduced which implies the in vivo antioxidant property of the extract. The expression of PPAR- γ in extract treated was found to be down regulated. Significant hypolipidemic effects produced in all the in vivo parameters indicate for the first time that leaves of this plant effectively attenuates HFD- induced obesity, atherosclerosis as well as hepatic steatosis by inhibiting adipocyte formation.

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