PRODUCTION, PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF BIOSURFACTANT PRODUCED BY CANDIDA TROPICALIS VEMS 27

Author(s): Thenmozhi M, Mani P and Boominathan M

Abstract: Nowadays, biosurfactants are one among the most important biochemical compound required for many industrial applications. So, the need for medium optimization is significant for enhanced production, this study used a potential biosurfactant producing estuarine yeast, Candida tropicalis VEMS 27 to accomplish the stated aim. This study optimized various physicochemical factors under shake flasks conditions and showed enhanced biosurfactant production at 42 h incubation with 2% of glucose, 1.5% of ammonium nitrate as carbon and nitrogen sources with pH 7, 35°C temperature and 10pp salinity. Under standardized conditions, the large-scale production was carried out and the biosurfactant was extracted with acid precipitation procedure and purified using silica gel column chromatography. The final dry weight of the purified biosurfactant was accounted as 4.85g/L and was biochemically characterized as glycolipid using TLC method. Thus, from the present study, the estuarine yeast C. tropicalis VEMS 27 proved the enhanced production of the glycolipid biosurfactant which is ideal for the industrial scale production and possible for the development of many industrial applications.

PAGES: 810-816  |  31 VIEWS  39 DOWNLOADS