IN VIVO AND IN VITRO EVALUATION OF TEPHROSIA VILLOSA FOR ANTI-DIABETIC PROPERTIES

Author(s): Ramesh. C* and Prameela Rani A

Abstract: Objective: The objective the present work was to investigate in vivo and in vitro anti-diabetic potentials of methanol extract of Tephrosia villosa against alloxan induced diabetes in albino rats. Methods: For in-vivo evaluation, diabetes was induced in albino rats by administering a single dose of alloxan. The study was designed test the acute effect of methanol extract of Tephrosia villosa (TVME) to reduce blood glucose in OGTT. The chronic study of 21 days was performed against diabetic rats and blood glucose was determined at 1st, 7th, 14th and 21st day. In chronic in vivo study, serum concentrations of insulin, urea, creatinine, total cholesterol, triglycerides, ALT and AST were also estimated at 21st day. The in vitro α-glucisidase inhibitory activity and α-amylase inhibitory activity were performed and IC50 values of extract was determined. The glucose up take by rat hemi-diaphragm model was also used test potentials of the extract to increase utilization of the glucose by tissues. Results: In OGTT, standard glibenclamide and TVME at 400mg/kg treated anomals have shown significant reduction in blood glucose at 90 mins but at 120 mins, blood glucose level was significantly reduced in glibenclamide and TVME at 200mg/kg and 400mg/kg treated animals compared to diabetic control group. In chronic model the methanol extract effectively, reduced blood glucose levels (P<0.001) at 14 th and 21st day of study in therapeutic groups and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduce concentrations of SGOT, triglycerides, cholesterol and urea in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial effects of the extract in diabetes. The change in concentrations of SGPT and urea were less significant (P>0.01).

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