Pancreatic-Lipase Inhibitory and Radical Scavenging Activities of Antheraxanthin-Di-Acetate

Author(s): Nagashree Shamarao, Syeda Hajira Banu and Mukunda Chethan Kumar

Abstract: Aim: To study pancreatic lipase inhibitory and antioxidant activities of antheraxanthin- di-acetate (ADA) synthesized from antheraxanthin (AX) which is extracted from orange juice. Methods: Extraction of antheraxanthin from orange juice, purification by TLC and synthesis of ADA from the extracted AX and analysis of radical scavenging activity by 2-Deoxy D-ribose assay and pancreatic lipase inhibitory activity by PNPP method. Results: The yield of AX was 2.36mg/g of orange pulp and ADA was 0.03mg/ml. Wavelength scan of AX and ADA showed  max of 510nm and 440 nm respectively. The percentage of inhibition of hydroxyl radicals, in standard BHA was 88.88%. Whereas, AX showed inhibition of 56.25% and ADA inhibited by 74.35% respectively. Percentage of Pancreatic lipase inhibitory activity of AX and ADA was about 12.53 and 69.57 in 100μl of sample respectively. Conclusion: ADA has better antioxidant and lipase inhibitory activities when compared with AX. Hence with further investigation, it can be developed as a potent anti-obese drug.

PAGES: 406-411  |  39 VIEWS  41 DOWNLOADS