Green Tea Extract-Propitious Approach to Combat Urinary Tract Pathogens

Author(s): S. Valli* and M. Divya

Abstract: Green tea is one of the most widely consumed beverages in the world and is currently perceived as a healthy drink now gaining worldwide popularity as a drink that is important in preventive medicine. It is derived from non-fermented leaves of the Camellia sinensis plant. Hence in the present work the antibacterial activity of green tea extract to combat urinary tract pathogens was assessed. Samples of urine were collected from hospital and processed for the isolation of pathogenic bacteria. Urine samples were processed and based on their morphological and biochemical characteristics the isolates were identified The effect of green tea extract was investigated against urinary tract pathogens. Ethanolic extracts of green tea were prepared by soxhlet extraction method. The antibacterial activity of ethanolic extracts were evaluated against pathogens by disc diffusion method. The maximum zone of inhibition was observed in Proteus mirabilis showing 39mm at 1mg/ml concentration followed by minimum zone of inhibition was observed in Escherichia coli (17mm) at the concentration of 1mg/ml. The phytochemicals occurring in the various solvent extracts of Camellia sinensis leaves were analysed quantitatively by phytochemical screening. The major phytochemicals found were saponins, flavanoids, alkaloids, proteins and steroids. GC-MS analysis was carried out on the ethanolic extract of Camellia sinensis and 10 different compounds were identified. The chromatogram revealed 15 peaks in the retention time range 10 to 30 minutes. The largest peak at 27.551 min with 7.54% area was identified as Tetracosane followed by Heptocosane. with 4.76% area at 26.099 minute. Screening of Camellia sinensis by GC-MS confirmed the plant to be a potential source for bioactive substances that supports several pharmaceutical uses and therapeutic value.

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