Human Dental Caries Pathogen Streptococcus Mutans affect Chitin Synthesis and that Restored by Antibiotics in the Silkworm, Bombyx mori

Author(s): M. Likhith Gowda, P. Shubha, K. Byrappa and H. B. Manjunatha

Abstract: Human dental caries pathogen Streptococcus mutans induced silkworm (Bombyx mori L.) disease model was used to examine the bactericidal effect on the cuticle chitin synthesis and its restoration due to antibiotics - Advent and Taxim that are clinically advocated to cure pulpal infections caused due to dental caries. Experimental data of the present investigation revealed that S. mutans infection distorted the synthesis and precipitation of chitin in the silkworm cuticle. Compared to control batches, a reduction of 35.50 and 39.83% of chitin was noticed in the larvae inoculated S. mutans through haemocoel and peroral routes respectively. Interestingly, antibiotics administered through the same routes accelerated a remarkable recovery of 33.8 (haemocoel) and 65.25% (per-os) of chitin respectively for Advent, while it was 21.77 and 46.75% for Taxim antibiotics. Thus, it is proven for the first time that inoculation of S. mutans both the routes were successful by way of inhibiting chitin synthesis and prepetition in the cuticle of the larvae and restoration of chitin synthesis as a response to antibiotics differs between two antibiotics, which are concentration and dose-dependent.

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