Phytochemical Analysis and in vitro Anti-Inflammatory and Anti-Bacterial Activities Curcuma Pseudomontana J.Graham

Author(s): Pinky Pavani, Yashasvi Jagapathi, Ram Mohan Manda*, Baba Shankar Rao Garige and Vasudha Bakshi

Abstract: Aim: The present study is aimed to evaluate the Phytochemical analysis and In-vitro Anti-inflammatory and Anti-bacterial activity of Curcuma pseudontana J.Grahm. Materials and methods: In the present investigation, the rhizomes of curcuma pseudomontana powder was extracted by successive soxhlation extraction method with Ethyl acetate and Methanol. The ethyl acetate extracts responded positively to all the tests for carbohydrates and also to the tests for flavonoids and glycosides and Methanolic extracts produced positive test for the presence of carbohydrates, proteins, steroids, flavonoid glycosides, tannins and phenol compounds. The extracts were used for testing the in-vitro anti-inflammatory activity by using albumin denaturation assay, proteinase inhibitory activity at a concentration of 20,40,60,80 and 100mg/ml and Anti-bacterial activity against two gram positive microorganisms (Bacillussubtilis and Staphylococcus aureus) and two gram negative microorganisms (Salmonella typhi and Escherichia coli) at concentrations 100 mg/ml and 200 mg/ml by adopting cup plate method. Results: The extracts exhibited significant in-vitro anti-inflammatory effect and inhibited the growth of both Gram positive and Gram-negative microorganisms at 100 mg/ml and 200 mg/ml concentrations. Conclusion: The findings of this study showed that the effectiveness of methanol extract shows more Anti-inflammatory and Anti-bacterial activity compared to ethyl acetate extract. Because of methanol extract contains more bioactive compounds comparatively then ethyl acetate extract and bioactive components justifying its traditional use.

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