Phytochemical Finger Printing of Abutilon indicum Extract using Chromatography Techniques and Bioautography Analysis of Active Compounds (Research Article)

Author(s): A. Anandhaguru*, J. Gokul, S. Lokesh, A. Sangeetharajan and N. G. Ramesh Babu

Abstract: Plants are capable of producing and synthesizing a large group of organic compounds known as primary and secondary metabolites and which are used primary health care. In this study the antimicrobial activity of the medicinal plant Abutilon Indicum was studied. The Soxhlet method is used to obtain Abutilon Indicum extract. Phytochemical analysis identifies the presence of bioactive compounds like tannins, saponins, flavonoids, alkaloids, protein, steroids, anthraquinones. Thin Layer Chromatography (TLC) indicates the separation of different compounds from the extract. Gas chromatography and mass spectrometry (GC-MS) controlled the compositions and resulted in identification of 27 compounds. From High performance liquid chromatography (HPLC) the flavonoid (rutin- 1.56 mg, quercetin-6.44 mg, gallic acid-2.91 mg) was quantified and identified. By using Bioautography, the antibacterial activity of the Abutilon Indicum extract was studied by using E. Coli, Salmonella sp., Staphylococcus sp., Vibrio sp. This earlier report can serve as an important platform for the development of further safe and effective natural medicines.

PAGES: 46-53  |  176 VIEWS  213 DOWNLOADS