Cytotoxic Evaluation and HPLC Profiling of Saraca asoca Ethanolic Extract for the Treatment of Endometriosis (Research Article)

Author(s): R J Reshmi Nair, S Lakshmi, K P Suja and K K Sabu

Abstract: This study investigated antioxidant activity of Saraca asoca extract using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and phytochemical analysis to evaluate the presence of secondary metabolites. In-vitro cytotoxic analysis was investigated in MDA-MB-231 (non-estrogenic) and MCF-7(estrogenic) cell lines along with endometrial cancer cell line Ishikawa. Viability of the extract was tested in L929 normal cell line with MTT assay. Column chromatography was performed and its HPLC profile were evaluated for the presence of bioactive compound, catechin. The extract exhibited significant concentration-dependent antioxidant and cell line activities. The safety profile of the extract with 85.86% cell viability in 500μg shows its efficacy. Its effect in MCF7 with IC50 value (416μg/ml) and MDA-MB-231 (518μg/ml) and prominent activity in Ishikawa cell lines (122μg/ml) indicate its significant use for endometriosis treatment. In the present study, DPPH activity HPLC profile and cytotoxic effects of the extract significantly substantiated the utility of the plant for endometriosis treatment.

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