Volume 12, Issue 4, October 2022
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Simultaneous Evaluation of Anti-Hypertensive agents by Using Stability Indicating RP-HPLC Method in Bulk and Tablets (Research Article)
Author(s): Malladi Srinivas Reddy* and Patnamshetti. Manasa
Abstract: The present article focuses on development of sensitive, simple, precise accurate and in expensive stability indicating RP-HPLC method using binary solvents for the simultaneous estimation of Amlodipine and Atenolol contents In Depisal a tablet. the separation was done with C18 water column come with the sizes 150×4.6mm&5μm with Acetonitrile&Na2HPO4 (PH5.2) merger in ratio 50;50(v/v), as a mobile phase with 1.0ml /min flow in isocratic type. The detection was done at 228 nm accompanied by quantification. The minutes of retention time for Amlodipine and Atenolol were 2.786 and 2.106, respectively. The Amlodipine 2.5-7.5 μg/ml and Atenolol 25-75 μg/ml were reported for linear responses. The method was precise and robust with LOD of ALD 0.025 μG/ml And ATN 0.098 μg/ml, respectively. The LOQ for ALD and ATN Were 0.082 μg/ml and 0.327μg/ml, respectively. Drugs were subjected to stress conditions in acidic, alkaline, peroxide of 30 % potency, photolytic thermal and hydrolytic. The method for this simultaneous estimation was found to be accurate, precise with precise values of ATN and ALD were 0.336% and 0.098% respectively Assay percentiles (accuracy) for ATN&ALD were 98.63 % and 99.55%, respectively.
PAGES: 188-196 | 38 VIEWS 85 DOWNLOADS
How To Cite this Article:
Malladi Srinivas Reddy* and Patnamshetti. Manasa. Simultaneous Evaluation of Anti-Hypertensive agents by Using Stability Indicating RP-HPLC Method in Bulk and Tablets (Research Article). 2022; 12(4): 188-196.