Development and Validation of RP-HPLC Method For the Simultaneous Estimation of Ramipril and Olmesartan Medoxomil (Research Article)

Author(s): K.Pravalika, Sashmitha Samuel.B*, M.Bindu and Sravanthi Vanama

Abstract: An RP-HPLC method was developed for the simultaneous estimation of Ramipril & Olmesartan medoxomil in bulk and tablet dosage forms using Hypersil C18 (4.6x250mm,5μm) column. 0.01M Potassium dihydrogen Phosphate pH 3(adjusted with orthophosphoric acid): acetonitrile (40:60) is used as the mobile phase. 228nm is used as the wavelength of detection. Linearity range is 5μg/ml to 25μg/ml for Ramipril & 20μg/ml to 100μg/ml for Olmesartan medoxomil. Retention times for Ramipril & Olmesartan medoxomil were 2.837 & 4.066 respectively. The developed method was validated as per ICH guidelines and the developed method was found to be precise, accurate, sensitive, and economical.

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