Estimation of a New Gabapentin Derivative in Capsule Dosage Form by New Validated UV Spectrophotometric Method (Research Article)

Author(s): Deepthi Akanksha J, Srinija A, Sandhya V, Vivek Sagar P and Sirisha K*

Abstract: A new simple, sensitive, and economic spectrophotometric method has been developed and validated for the determination of a new gabapentin derivative in pure form and pharmaceutical preparations. The method is based on the reaction between the amino group of gabapentin with benzene sulphonyl chloride to form the gabapentin derivative (sulphonamide) via addition-elimination mechanism. The new gabapentin derivative was prepared by two different methods using sodium carbonate (method 1) and sodium hydroxide (method 2). The colourless product obtained was analysed by TLC. In UV-spectrophotometry, its absorption maximum was found to be 275nm in ethanol. The linearity range for the new gabapentin derivative was found to be 2-10 μg/ml. Accuracy was performed at three concentration levels of 50%, 100% and 150% and the respective percentage recoveries were found to be 1.34,1.06 and 0.68. Precision results were found to be within the limits of acceptance criteria and the method was found to be robust with % RSD less than 2.0. The LOD and LOQ values were found to be 0.28μg/mL and 0.86μg/mL respectively.

PAGES: 26-35  |  138 VIEWS  169 DOWNLOADS