In vitro Propagation of Anther Culture of Datura (Datura stramonium. L) Using MS Media (Research Article)

Author(s): Jamilu Danjuma Koko, Kasimu Shehu, Adamu Aliyu Aliero, Muhammad Danladi Abubakar Bunza, and Ja’afar Umar, aFederal University Birnin Kebbi. Nigeria P.M.B. 1157 Birnin Kebbi and bUsmanu Danfodiyo University Sokoto, Nigeria P.M.B. 2346 Sokoto

Abstract: Datura stramonium L. is a medicinal plant that makes tropane alkaloids and generates secondary metabolites during in-vitro culture. The study aimed at using standardizes explant (anther) surface sterilization to generate a virus free plant. A different culture of Datura stramonium was in-vitro propagated in MS basal medium with the addition of 30 g/L sucrose, 8 g/L agar, various mixtures of 2, 4, and Dichlophenoxyacetic acid (at three levels, 0, 1, and 2 mg/L), and kinetin (0, 0.25, 0.5, and 1 mg/L). The calli were then moved to regenerative media that contained BAP alone (at three levels, 1, 2, and 3 mg/L), or in conjunction with NAA (at four levels, 0, 0.02, 0.2, and 1 mg/L). The newly grown shoots were planted in soil with IBA at three different concentrations (0.5, 1, and 1.5 mg/l). The best media for inducing callus from anthers as explants were discovered to contain 2 mg/L 2, 4-dihydroxyacetic acid + 0.5 mg/L kinetin and 2 mg/L of 2, 4-D alone. Furthermore, the best hormonal treatments for shoot regeneration from calli of anther explants were discovered to be 3mg/L BAP+ 1mg/L NAA media and 2mg/L BAP+ 1mg/L NAA media. Additionally, the best method for rooting regenerated stems was found to be 0.5 mg/L IBA. Further research on leaves for generating a virus free plant should be conducted.

PAGES: 154-160  |  192 VIEWS  194 DOWNLOADS