CELL PROLIFERATION STUDIES ON HUMAN PROSTATE CANCER CELLS (PC3, DU145 AND LNCAP) USING CURCUMIN

Author(s): Anitha Joice*, J. Manju Bashini, S. Surabi and P. Senthilkumaar

Abstract: Curcumin, a major yellow pigment and spice in turmeric and curry, is a powerful anti-cancer agent. The antitumour activities of curcumin include inhibition of tumour proliferation, angiogenesis, invasion and metastasis, induction of tumour apoptosis, increase of chemotherapy sensitivity, and regulation of cell cycle and cancer stem cell, indicating that curcumin maybe a strong therapeutic potential through modulating various cancer progression. It has been reported that the anticancer effect of Curcumin against human prostate cancer cell line through Proliferating Cell Nuclear Antigen (PCNA) assay and colony forming assay. Different concentrations (1, 5, 10, 25, 50 and 100 μg/ml) of Curcumin were used. In PCNA assay, curcumin slows cancer cell proliferation and in colony forming study, colonies were stained with crystal violet and the number of clones in a given area was counted for each condition. This study witnessed that higher concentration of the curcumin gave a good result against cancer cell lines.

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