SIMULTANEOUS ESTIMATION OF IMIDAPRIL HYDROCHLORIDE AND HYDROCHLOROTHIAZIDE BY RP-HPLC

Author(s): Veditha.K* and Sailaja.B

Abstract: A simple isocratic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and subsequently validated for simultaneous estimation of anti-hypertensive drugs, Imidapril(IMP) and Hydrochlorothiazide(HTZ) in tablet formulation. Quantification was carried out by using an isocratic mode on HPLC of Phenomenex Luna (C18 250 X 4.6 mm, 5 microns) with flow rate of 1.0 ml/min and UV detection at 218nm. The separation was carried out using a mobile phase consisting of phosphate buffer: acetonitrile: methanol (58: 25: 17 v/v) and adjusted the pH to 2.5. The retention time for IMP and HTZ was found to be 6.243min and 2.370min respectively. A linear response was observed over the concentration range of 5-30 mcg/ml each for the assay of IMP and HTZ. The results of analysis were validated and recovery was found to be  98%. Hence the proposed method was found to be accurate, precise, reproducible and specific and can be used for simultaneous analysis of these drugs in pharmaceutical formulation.

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