ACTINOMYCETES DIVERSITY IN FIVE FRESH WATER SYSTEMS OF PUDUKKOTTAI, TAMIL NADU AND THEIR ANTIMICROBIAL ACTIVITY
Author(s): Saravanan S, Sivakami R* and Prem Kishore G.
Abstract: Actinomycetes are excellent elaborators of biotechnological products such as antibiotics, industrial enzymes and other bioactive compounds. Emergence of multiple drug resistant pathogens implies the need to search for new and novel antimicrobials and the discovery of new molecules from actinomycetes has marked an epoch in antibiotic research and subsequent developments in antibiotic chemotherapy. Sediment samples were collected from five different ponds of Kottaipattinam region, Pudukkottai District situated near the Bay of Bengal. Actinomycetes strains were isolated from the air-dried sediment samples by applying serial dilution and spread plate technique on three different media such as Glycerol-Arginine Agar, Starch-Casein Agar, and Starch-Nitrate Agar. Different media were used in order to compare the recovery on different media as well as to obtain maximum diversity of actinomycetes strains. The test organisms used were bacteria such as Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Bacillus cereus, Vibrio cholerae, Salmonella paratyphi and Staphylococcus epidermidis. The test fungus/yeast used were Aspergillus niger and Candida albicans. The test organisms were collected from Government Hospital, Tiruchirappalli, Tamil Nadu. In the present work, the antibacterial activity of actinomycetes strains were checked by using three different media (such as Glycerol Argine Agar (GAA), Starch- Casein Agar (SCA) and Starch Nitrate Agar (SNA) and three methods (like cross streak method, spectra-plate method and well diffusion method). However, the strains showed antibacterial activity only by well diffusion method on Glycerol-Yeast Extract Agar for bacteria and Potato Dextrose Agar for fungi. Thus, it is concluded that the substances repressing these strains were accumulated only in the mycelium and not in the medium.
PAGES: 126-130 | 115 VIEWS 80 DOWNLOADS
How To Cite this Article:
Saravanan S, Sivakami R* and Prem Kishore G.. ACTINOMYCETES DIVERSITY IN FIVE FRESH WATER SYSTEMS OF PUDUKKOTTAI, TAMIL NADU AND THEIR ANTIMICROBIAL ACTIVITY. 2018; : 126-130.