PRELIMINARY PHYTOCHEMICAL SCREENING, IN-VITRO EVALUATION OF TRIFOLIUM REPENS L. FOR ANTHELMINTIC AND ANTIBACTERIAL ACTIVITIES

Author(s): H. Ramana*, R.Vasanthi, P.Annapurna, M.Prashanthi and V.Mamatha

Abstract: The present work was conducted to investigate the preliminary phytochemical studies, antihelminthic and anti-bacterial activities on the leaf extracts of Trifolium repens L. family Fabaceae. The anthelmintic activity was evaluated on adult Indian earthworm Pheritima posthuma due to its anatomical and physiological resemblance with the intestinal roundworm parasites of human beings. The antibacterial activities of the extracts of Trifolium repens L. were performed by agar cup plate method. Preliminary phytochemical screening of petroleum ether, ethyl acetate, methanol and aqueous extracts of leaves of T. repens L. revealed the presence of carbohydrates, saponins, phytosterols, triterpenoids, tannins, phenolic compounds, alkaloids and amino acids. All the tested extracts showed mild to moderate anthelmintic activity and antimicrobial activity, albendazole and amikacin sulfate were employed as reference standard for anthelmintic and antibacterial activities respectively. Among the tested extracts the aqueous and methanolic extracts were found to possess potent anthelmintic activity while ethyl acetate extract showed moderate activity. Whereas petroleum ether extract showed most promising antibacterial activity, while ethyl acetate showed moderate activity. The present study shows the potent anthelmintic as well as antibacterial activity of T. repens L. that was good in their action against the worms.

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