SCREENING AND PRODUCTION OF ALKALINE PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM SOLAR SALTERN

Author(s): Jeya. K. R*, Veerapagu. M, Vetrikodi. N, Priya. R and Latha. G

Abstract: Halophiles are predominant organisms which are present in the hypersaline environment saltern ponds. They produce a variety of industrially important bioactive compounds including protease enzymes. Soil samples were collected from solar saltern of Vedaranyam, Tamilnadu. A total of 12 halophilic bacteria HPB1 –HPB12 isolated were screened for proteolytic activity by plate assay showed that seven isolates exhibited proteolytic activity by zone formation around the colonies. Isolate HPB2 produced high amount protease enzyme 35.4U/ml than the other seven isolates were identified as Bacillus megaterium. The effect of incubation time, medium pH, temperature, NaCl concentration and carbon source source for the protease production was investigated. Protease enzyme production by Bacillus sp was maximum at 40 hrs of incubation period 86.5 U/ml, pH 8 (107.2Uml-1), temperature 40oC (98.3 Uml-1), Nacl 2M (102.8 Uml-1). and glucose (124.5 Uml-1) as carbon source.

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