COMPARATIVE STUDIES OF APIXABAN IN BULK AND ITS FORMULATIONS BY UV-SPECTROSCOPY (ZERO DERIVATIVES AND AREA UNDER CURVE)

Author(s): Annareddy Gari Radhika, Anurati Singh, Akkenapally Sowmya, Akiful Haque M, Vasudha Bakshi, Narender Boggula*

Abstract: A simple, rapid, precise and highly selective spectrophotometric method was developed for estimation of Apixaban in tablet dosage form by Zero Order Derivative and Area Under Curve. Zero order derivative and Area Under Curve methods involve the measurement of absorbances of Apixaban at the wavelength of 279 nm and 269-289 nm respectively. Methanol was used as solvent. Linearity was observed in the concentration range of 5-25 μg/ml for Apixaban. The accuracy of the Zero order derivative method and AUC were confirmed by recovery studies of tablet dosage forms and were found to be 100% and 100% for Apixaban respectively. The Zero order derivative method and AUC showed good reproducibility and recovery with % RSD less than 0.790% and 0.988%. The LOD for Zero order derivative method and AUC of Apixaban were found to be 0.23 μg/ml and 0.335 μg/ml and LOQ for Zero order derivative method and AUC of Apixaban were found to be 0.713 μg/ml and 1.015 μg/ml respectively. Thus, the proposed method was found to be rapid, specific, precise, accurate and cost-effective quality control tool for the routine analysis of Apixaban in bulk and tablet dosage form. Drug stability studies have been determined for the formulation under specified conditions and it was found stable.

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