Screening of Phytochemicals in Methanolic Extract of Dictyota Dichotoma (Huds.) Lamouroux From Koothankuzhi in the South East Coast of Tamil Nadu, India

Author(s): John Peter Paul J*

Abstract: The present study was carried out to screen the phytochemicals present in the methanolic extract of Dictyota dichotoma (Huds.) Lamouroux collected from Koothankuzhi in the south east coast of Tamil Nadu, India. The phytochemical screening of methanolic extract was screened using the standard procedure for UV-Visible spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed the compounds separated at the nm of 450, 500, 534, 600, 655, 700, 750, 800, 850 and 900 with the absorption 4.000, 3.101, 2.048, 2.075, 3.311, 0.750, 0.101, 0.024, 0.006 and 0.003 respectively. The qualitative HPLC fingerprint profile displayed eight compounds at different retention time of 2.020min, 2.187min, 2.303min, 2.637min, 2.790min, 3.027min, 5.430min and 6.550min. The result of FTIR analysis was found the presence of functional groups such as nitrates, phenyl (C-O and C-H bonds), aliphatic ketones, sulphonic acid ester, oximes (C=N stretching), aromatic aldehyde, tertiary amine salts, alkanes, methoxy and hydroxyl group.

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