Optimization of Cyclodextrin Glucano-Transferase by Bacillus Cereus Isolated from Semi Arid Soil, Kuchchh, Gujarat

Author(s): Dhaval Prajapati, Rajesh Chaudhari, Vikram Solanki and Shreyas Bhatt

Abstract: Cyclodextrin glucanotransferase (CG-Tase) is a commercially important enzyme which catalyses the formation of cyclodextrins (CDs) from starch belonging to the amylase family. The optimization enzyme cyclomaltodextrin glucano transferase was carried out from semi arid region of Kachchh Gujarat different enzyme activities and optimization of cyclodextrin glucano-transferase, with different physicochemical Parameter were Temperature effect was observed 35°C with 42.02 U/ml enzyme activity, were the most active at pH 7.0 reported 44.28 U/ml, at highest incubation at 96 hrs, activity was 39.68 U/ml. stood with different inoculum at 3% of inoculum, 54.38 U/ml activity verified highest. Different cations zinc express highest activity 36.98 U/ml. In case of different carbon source starch prompt highest activity at 39.28 U/ml. while Response surface methodology and standardization error to estimate to the mean value of observed response.

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