Isolation, Morphological Characterization and Antibacterial Activity of Endophytic Bacteria from Medicinal Plants of Muthathi Wild Life Sanctuary, Mandya, Karnataka, India

Author(s): Suresha. S* And M. Jayashankar

Abstract: Medicinal plants play a vital role in providing crucial health care to human populations. These plants are very good source of endophytic bacteria with their potential to produce the bioactive compounds having antibacterial activity against human pathogens. Endophytes are microorganisms that are existing in living tissues of several plant tissues and these are chemical synthesizers have been founding regular association without apparently causing any symptom of diseases. The purpose of this study was to isolate the endophytic bacteria along with their initial characterization from few selected medicinal plants and to evaluate antibacterial activities against human pathogens. A total of 84 endophytic bacteria were isolated and identified by morphological characterization from the leaves of few selected medicinal plants of Cauvery Wild Life Sanctuary (Muthathi), Mandya. These isolates were subjected to antibacterial activity against eight clinically significant pathogenic bacteria. Out of this, 8 bacterial endophytes exhibited the antibacterial activity against S. aureus, E. coli, B. subtilis, P. aeruginosa, Shigella, K. pneumoneae, P. mirabilis and L. monocytogens by using standard protocol of agar well diffusion method. Aloe vera (L) Burm.f, Cassia auriculata L, Madhuca longifolia J.F.Macbr, Origonum majorana L., Sesbania grandiflora L, Vitex Negundo L, Withania Somnifera L Dunal, plants isolates were inhibit the effectiveness antibacterial activity with zone of inhibition. Thus, this study exposed bacterial endophytes with the capability of producing antimicrobial elements of pharmacological importance.

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