Production and Characterization of Bioplastics from Marine Bacterium Bacillus Cereus

Author(s): B. Sundarapandiyan*, G. Aarthi and S. Jayalakshmi

Abstract: Biopolymers are generated from renewable natural sources and are often biodegradable and nontoxic. A fully biodegradable polymer is defined as a polymer that is completely converted by living organisms, usually microorganisms, to carbon dioxide, water and humic material. The aim of the present investigation was production and optimization of bioplastic by using the marine bacterium Bacillus cereus. Mangrove soil samples was collected from Vellar estuary and serially diluted. The diluted samples were inoculated in the surface of Mineral agar medium by spread plate technique. Totally 16 different strains were isolated from that 10strains showed the accumulation of PHB in the nitrogen deficient medium. Among which Bacillus cereus showed the maximum PHB accumulation. In the present study temperature showed the profound influence on the PHB production and optimum temperature for maximum production was found to be 35C. pH also showed influenced on the growth and the optimum pH-7 for PHB production. Salinity-0.5% was optimum for PHB production. In the present study, sucrose and peptone was found as the best carbon and nitrogen source for maximum growth. Bacillus cereus can be used in industries for large scale production and also alternate to produce truly biodegradable polymers.

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