Box-Behnken Optimization and Characterization of Ultrasonic-Assisted Extraction of C-Phycocyanin from Spirulina platensis

Author(s): Chinnappan Sudhakar, Arumugam Sengottaiyan, Periyasamy Thiyagarajan, Kandasamy Selvam* and Thangaswamy Selvankumar**

Abstract: C-phycocyanin is a natural pigment widely used as food, solar cells, feed additive, cosmetic and pharmaceutical products. The current study investigates the simple, economic and rapid ultrasonic-assisted extraction (UAE) procedure was developed and response surface methodology (RSM) based to optimize C-phycocyanin from Spirulina platensis. Several variables that can potentially use to extraction of pigments, namely solid: liquid ratio, ultra-sonication time (h), microwave power and the number of cycles were optimized by means of a RSM approach. The predicted optimal conditions for the highest pigment yield were found at 1:10 (g/mL) solid: liquid ratio, 12.5 min sonication time, 100% microwave power, and 5 cycles. FT-IR spectroscopy analysis used to identify the chemical changes of the before and after pigment extraction. Therefore, this methodology could be used for extracting C-phycocyanin from algae in a faster and effective manner.

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