RP-HPLC Method Development and Validation of Chrysin in Bulk and Marketed Formulation

Author(s): K. Vajramma, Shaheen Begum*, SK Arifa Begum, Jayasree and K. Bharathi

Abstract: Chrysin has interesting activity profile. It exerts anti-inflammatory, anticancer, hypoglycemic, antioxidant and anti-ageing properties. In this study a novel, simple, precise and linear reveres phase high performance liquid chromatography method (RP-HPLC) is described for bulk and marketed formulation of chrysin. Methanolic solution of chrysin was injected (injection volume 20μl at a flow rate 1ml/min) in reversed phase column using acetonitrile and methanol (65:35V/V) as mobile phase. Detection was set at 268 nm (λmax). The method was found to be linear with correlation coefficient of 0.99 (0.2-6.4 mg of chrysin). Using this method it was possible to detect chrysin at 3.39 min of retention time and method is validated by determining all the validation parameters.

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