Isolation and Characterization of Agarase Producing Bacteria from Marine Ecosystem of South Gujarat

Author(s): Panchal H.K *

Abstract: Marine ecosystem is the least explored ecosystem of the earth which contains microbes capable of producing different enzymes. This study was focused on isolation and characterization of agarase producing microorganisms from marine water, as many marine algae are great source of agar-agar. In present study marine samples were collected from Tithal, Danti and Daman beaches from South Gujarat, India. Enrichment and plating were done on Minimal mineral salt (MMS) agar medium, 30 isolates were obtained from primary screening. Secondary screening was performed for confirmation of Agarase production and 3 maximum activity giving isolates C-17, C19 and C-29 were selected for further characterization study. During characterization all 3 isolates gave optimum activity at pH 5, temperature 45℃, and 4% NaCl concentration. The partial purification of crude enzyme was performed by ammonium sulphate precipitation and good activity was found at 80% saturation.

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