Pharmacognostic and HPTLC Evaluation of Leaves and Roots of Trianthema portulacastrum L.

Author(s): Sakkeena A., Anitha John*, Manju K. C, Gayathri Devi V, Neethu Kannan B and Kanagarajan A

Abstract: Trianthema portulacastrum L. (TP) belongs to the family Aizoaceae. It is rapidly growing, much branched, succulent, prostrate and annual terrestrial weed. The plant parts such as leaves, fruits and roots are used for various medicinal purposes. Some traditional uses reported are as alterative cure for bronchitis, heart disease, blood anemia, inflammation, piles, ascites and throat troubles. The root paste applied to the eye cures corneal ulcers, itching, dimness of sight and night blindness. Various pharmacological activities of the plant have been reported. The present work deals with the physico-chemical, botanical and HPTLC studies of leaf and root of TP. The physico–chemical parameters such as water soluble extractive, alcohol soluble extractive, loss on drying at 105℃, total ash, acid insoluble ash, pH (10% solution) and volatile oil were determined according to standard methods. The macroscopic, microscopic and powder microscopic characteristics of leaf and root were determined. HPTLC studies of chloroform extracts of leaf and root of TP were conducted at 254 nm, 366 nm and 575 nm after derivatisation using vanillin- sulphuric acid reagent and the results were documented. The total ash content and the solubility in alcohol and water for leaf are higher than that of root. The Physico-chemical parameters, Macroscopical, Microscopical and Powder Microscopical characters and the developed HPTLC fingerprint profiles obtained from this study can be used as reference standards in laying pharmacopoeial standards of the two plant materials.

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