Food Industry Waste Material as A Growth Medium for Rhizobium

Author(s): Ashok Kumar Singh, Gauri Singh and Gaurav Yadav

Abstract: Ever increasing industrialization have considerably increased the rate of water pollution. the management of waste is a major challenge for administrators and planners. To overcome those problems, there is an alternative process for using these wastes, as a source for cultivation media of micro-organisms. The present work was aimed to designing a food industry waste water based medium that included mushroom and dill, as a substitute for costly commercial media for growing rhizobium. Growth and optimization parameters of two strains of rhizobium namely R1 and R2 at different concentrations of food waste was observed. Growth of both the strains were highest in 90% waste. Growth effects of strains were observed at 90% food waste along with different synthetic media and was found to be superior to standard media (TY and YEM) used for Rhizobium. Media was optimized using 90% food industry waste along with different concentrations of yeast extract (1-7 g/l) and mannitol (1-7 g/l) in terms of OD at different intervals of time i.e. 24, 48 and 72 hours. The maximum growth was observed in 4g/l in yeast extract and mannitol in both the strains. Bacteria were grown on the waste water medium which produced effective results and Competed with standard media like YEM and TY. Hence, this study led to a new and efficient medium for bacterial cultures as well as decrease in the waste disposal expenses and efforts.

PAGES: 144-155  |  231 VIEWS  336 DOWNLOADS