Biotransformation of Pyrazole Chalcones By Conidiobolous Species Isolated from Detritus Vegetable Waste

Author(s): Nisar Ahmed* and Shaikh Abdul Baseer

Abstract: Microorganisms showed their potential ability to degrade bioactive natural and synthetic compounds. Conidiobolus Sp. isolated from detritus vegetable waste was used for biotransformation of pyrazole chalcones under laboratory conditions on MSM (minimal salt medium) containing malt extract-0.1%, yeast extract-0.2%, glucose-0.5%, peptone-0.2% medium at 30oC for 10 days. After incubation sample was harvested and analysed by TLC, 1H NMR, LR MS. The product which formed in the bio reduction reaction using Conidiobolus species was confirmed from spectral analysis was 1-(5-chloro-2-methyl-phenyl)-3-(5-chloro-3-methyl-1-phenyl-4H-pyrazol-4-yl) propan-1-one (ie reduced pyrazole chalcones). The biotransformation of pyrazole chalcone by Conidiobolus sp. reaction was regioselective, showing alternation of C-C double bond of the olefin and do not alter the carbonyl double bond of α, β unsaturated ketone system.

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