Comparative Antimicrobial Activity of Ethanolic and Aqueous Extracts of Asparagus resmosus

Author(s): Krishnadutt Pratihast, Rajneesh Kumar,* and Sudhanshu Kumar Bharti

Abstract: The present study was designed with the aim to evaluate and compare the effects of ethanolic (EtOH) and aqueous (H2O) extracts of Asparagus resmosus (satavar; Family: Asparagaceae) plant extract according to phytochemical aspects for antibacterial efficacies. Phytochemical screening of the plant extract showed the presence of different percentage of active phytochemicals such as alkaloid, flavonoid, phenolic, saponin, tannin etc. The phenolic content of ethanolic extract was found to be 9.4 mg GAL/g while 6.8 mg GAL/g per gram dry weight basis for aqueous extract. The flavonoid content of ethanolic extract in terms of quercetin equivalent was found to be 0. 38 mg/g while for aqueous extract it was found to be 0.46 mg/g. The IC50 value of A. racemosus in ethanol was found to be 0.2016 μg/ml while that in water was found to be 0.2248 μg/ml. The ethanolic extract of A. racemosus showed the maximum zone of inhibition (7.50 mm) against E. coli followed by Salmonella typhi (7.00 mm) and Bacillus cereus (7.00 mm). The extract of Asparagus racemosus showed the minimum zone of inhibition (6.0 mm) against Bacillus subtilis. The aqueous extract of Asparagus racemosus showed the maximum zone of inhibition (7.50 mm) against E. coli followed by Salmonella typhi (7.00 mm) and Bacillus subtilis (5.00 mm). The extract of Asparagus racemosus showed the minimum zone of inhibition (4.0 mm) against Bacillus cereus.The minimum inhibitory as well as bactericidal concentration of investigated plant extract against bacterial strains of E. coli has been found to be 250 μg/ml.

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