Quality Control and Standardization of Colchicum (Colchicum luteum Baker) Rhizome with the help of TLC, HPLC and HPTLC

Author(s): Syed Shariq Mian, Tajuddin, Sukirti Upadhayay and Mohd. Irshad Alam

Abstract: Background: Colchicum (Colchicum luteum Baker) is a well-known traditional medicinal plant used for therapeutic effects in Unani and Ayurvedic System of Medicine. It has been reported to have many therapeutic activities like anti-inflammatory, anti-rheumatic and anti-gout properties. It is also useful in the management of Sciatica, Headache, Wound healing, diseases of liver and spleen, Sexual debility. The present study aimed towards the evaluation of the parameters involved in the determination of the quality and purity of Colchicum luteum Baker. corm and its standardization. Material and Methods: Organoleptic characters, extractive values, ash values, phyto-chemical analysis, TLC, fluorescence analysis, HPLC and HPTLC profile etc. were the parameters used for the standardisation of the test drug. Result: Total ash values, water, alcohol, Ether soluble extractive values and volatile oil percentage was found to be 6.208%, 21.173%, 1.693%, 0.22%, and 1.60% respectively. TLC profile of Colchicum luteum Baker. shows 01, 03, 01, 04 and 10 spots in UV short, long wavelength and exposure in anisaldehyde-sulphuric acid at room temperature, 65oC and 95oC respectively. The HPLC of Standard Drug (Colchicine) pattern shows 10 peaks and the peak no.6 is major peak having area concentration and retention time as 99.45% at 3.19 min. While Crude Drug (Colchicum luteum Baker) shows 13 peaks and the peak no.2 and 1 are major peaks having area concentration and retention time as 62.28% at 3.20 min. and 33.76% at 2.85 min. respectively. Conclusion: The study will provide referential information for the good quality, purity and identification for the future batches of Colchicum luteum Baker.

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