Green Synthesis of Silver Nanoparticles and Antimicrobial Studies in Aqueous Bulb Extract of Eulophia graminea Lindl.

Author(s): Sriramulu Arava and Lokanatha Valluru*

Abstract: Development of environmentally benign methods for synthesis of nanoparticles is an evolving important branch of nanotechnology. In the present study we report the green synthesis of stable silver nanoparticles (SNPs) from aqueous bulb extract of Eulophia graminea. These green synthesized nanoparticles are characterized by using UV-VIS spectroscopy, Zeta potential, FTIR, XRD, and TEM with EDAX. The colour change from grey to brown is observed upon synthesis and 430 nm peaks obtained from UV-VIS spectroscopic analysis. Zeta potential at -31.2 mV, negative value indicates the high stability of nanoparticles. FTIR spectroscopic studies confirms that phenols and proteins of bulb extract is main responsible for capping and stabilization of these SNPs. Crystallographic studies from XRD indicated the SNPs are crystalline in nature and owing 50 nm size. High resolution and magnification studies with TEM analysis revealed that the nanoparticles are spherical in shape having the size range from 5 to 50 nm. EDAX pattern of synthesized SNPs showed 62.53 weight percentage of Ag metal in the sample indicate the purity of sample. Further, the antimicrobial studies of these green synthesized SNPs show high toxicity towards different bacterial isolates. The results revealed that the selected medicinal plant possess the potentiality towards the synthesis of narrow range nanoparticles also combat with the pathogens.

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