Phytochemical and Antioxidant Activities of Leaf extracts of Ocimum americanum

Author(s): Pooja Jaiswal, Alpana Yadav and Nishi Kumari*

Abstract: Presence of antioxidant activity, polyphenolic compounds and reducing potential activities were assessed in the extracts of Ocimum americanum. Extracts of dry and green leaves in different solvents (ethanolic, methanolic and aqueous) were used for phytochemical assays through standard methods. Maximum antioxidant activity was observed in methanolic extract of green leaf i.e. 335.461μg mL-1. Total phenolic content was maximum in ethanolic extract of green leaf (282.67±2.17mgg−1 of gallic acid equivalent) and total flavonoid content was maximum in methanolic extract of dry leaf (946.67 ± 0.88 mgg−1 of rutin equivalent). Aqueous extract of green leaf showed highest reducing potential. Positive correlations were observed between antioxidant activity and polyphenolic compounds (total phenolic and flavonoid content). Similarly, significant correlation was found between antioxidant activities and reducing potential.

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