Antibiotic Production and Its Antibacterial Activity Against Clinical Pathogens Isolated from Wounds

Author(s): Raut Jyoti, Sonune Yogeeta, Warudkar Radhika and Chavan Neha*

Abstract: Actinomycetes are slow growing, Gram positive filamentous microorganisms. Actinomycetes are believed to be an excellent source of producing ntibacterial substances with high commercial value. The Objective of this study is to determine antibacterial activity of antibiotic extracted from isolated Actinomycetes against pathogens of infected wounds and to characterize extracted antibiotic by FT-IR analysis. In this study, crude antibiotic was produced and extracted from Actinomycete i.e. Streptomyces sp. isolated from soil. Total Six bacterial cultures were isolated from wound infected patients. They were identified based on Biochemical and Cultural characterization according to Bergey’s Manual of Systematic Bacteriology as Micrococcus sp., Staphylococcus sp., Corynebacterium sp., Enterococcus sp., Escherichia coli, Proteus sp. The Antibacterial activity of crude antibiotic was studied against wound isolates by Agar well diffusion method. The highest zone of inhibition i.e.24 mm was found against Enterococcus sp. at 800 μg/ml concentration of crude antibiotic and lowest zone of inhibition was observed against Corynebacterium sp.i.e.14 mm at 200 μg/ml. The Antibiotic extracted from Streptomyces sp. showed good antibacterial activity against Gram positive isolates compared to Gram negative bacterial isolates.

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