Chemometrics Assisted UV-Visible Spectrophotometric Method for Simultaneous Estimation of Ascorbic Acid and Gallic Acid from Herbal Formulations

Author(s): K. Lakshmi*, G. Neelima and D. Balanagamani

Abstract: In this work, numerical methods, based on the use of spectrophotometric data coupled to Partial Least Squares (PLS) and Principal Component Regression (PCR) is evaluated for the simultaneous determination of Ascorbic Acid (ACA) and Gallic acid (GAA) in bulk and formulations. Spectra of ACA and GAA were recorded at concentrations within the linear range of 0-3 μg/mL and 0-6 μg/mL respectively and were used to compute a total of 35 synthetic mixtures comprising of 25 calibration and 10 validation sets between the wavelength range of 200 – 350 nm with the wavelength interval of 1nm in hydroalcoholic solution (Water: methanol in the ratio 1:1). The suitability of the methods was assessed by means of root mean squares error (RMSE) for calibration and prediction sets. The analytical performance of these methods were evaluated by means of percentage recovery data. These methods were successfully applied to ayurvedic formulations and the drugs were evaluated without any interference from other compounds. The proposed methods are simple, rapid and can be easily used as an alternative tool for routine quality control analysis.

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