Phytoconstituents Screening by GC-MS and Evaluation of In Vitro Gastroprotective and Antioxidant Activity of Ulva lactuca

Author(s): M. Sulthanabegam, S. Palanicand B. Senthilkumar

Abstract: Background: The discovery and development of antibiotics are among the most powerful and successful achievements of modern science and technology for the control of infectious diseases. Seaweeds are rich source of original and bioactive natural substances. In particular, Green seaweed, Ulva lactuca have been demonstrated to metabolize biomolecules with pharmacological potential. Aim: The ethanolic extract of collected alga, ulva lactuca was subjected to screen phytoconstituents, antioxidant activity and cytotoxicity assays. The bioactive compounds were identified by GC–MS analysis. Materials and methods: The antioxidant activity was carried out by reducing power assay, DPPH activity, superoxide and nitric oxide radical scavenging activity, evaluation of total antioxidant capacity of the extract. The phytochemical analysis was carried out as per standard methods and Agilent GC-MS 680 analysis performed using a fused silica capillary column. Cytotoxic activity also employed against gastrointestinal protection in cell line for three different solvent extracts. Results: Ethanolic extract of ulva lactuca showed higher antioxidant activity and the presence of phytochemicals like alkaloids, flavonoids steroids, tannins, terpenoids, phenol, carbohydrates and saponins and also possess cytotoxic activity in cell line, ethanolic extract showed highest activity. The GC-MS analysis provided different peaks determining the presence of 20 different compounds, among that 13 major compounds viz., Beta Carotene, Eicosatetraenoic acid Trans-Traumatic acid 1-Naphthalenepropanol, which exhibit the antioxidant, antibacterial, antimicrobial, anti-inflammatory, antiulcer and anticancer activity was present in this algae. Conclusion: In the present study it was concluded that the Ulva lactuca is a rich source of antioxidant and pharmaceutically important by the presence of various bioactive compounds used for the treatment of human pathogenic diseases.

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