Volume 9, Issue 3, July 2019

Evaluation of Anticancer Activity and In- Vitro Release Assay of a Polymeric Microsphere Formulation for Colon Targeted Delivery of 5-Fluorouracil using Biocompatible Natural Gum Katira.

Author(s): Saumen Karan, Debjyoti Naskar, Souvik Debnath, Tapan Kumar Chatterjee
Abstract: Development of anticancer drugs is fraught with challenges which go beyond the typical problems and issues of pharmaceutical research. The chemotherapeutic agent must be able to selectively kill or restrain the growth of neoplastic cells leaving healthy cells unharmed. These drugs are almost always highly toxic, especially to rapidly dividing normally.The present aim was to develop oral site-specific rate-controlled anticancer drug delivery to subdue systemic side-effects, and provide an effective and safe therapy for colon cancer with compressed dose and duration of therapy. The double emulsion, solvent evaporation (or solvent extraction) technique also called the water-in-oil-in-water (W/O/W) emulsion was used to encapsulate hydrophilic compounds, overcoming the limitations of a single oil-in-water emulsion. Drug release-retarding polymers are the key performers in designing a microsphere dosage form for oral sustained release formulation. The microspheres were then characterized for drug loading, entrapment efficiency, SEM (Scanning Electron Microscopy), FTIR (Fourier transform infrared spectroscopy). To verify the functionality of 5-FU loaded gum katira microsphere for sustained drug release systems, cell cytotoxicity and in-vivo anticancer activity were also tested. The drug release was checked in the physiological media of the gastrointestinal tract, i.e., simulated gastric fluid (SGF) and simulated colon fluid (SCF). Formulated and optimized polymeric microsphere of 5-FU using gum katira polymer own optimal physicochemical characteristics with a fine spherical particle, with a size of 320.75±5.73 μM, high drug entrapment efficiency (74.87±1.76 %), and satisfactory release pattern of the drug within a time range of 12 h. According to the MTT results, targeted microsphere exhibited good cytotoxicity and have productive anticancer activity on sarcoma180 solid tumor mice. Finally, we foretell that polymeric microsphere of 5-FU using natural gum katira could be a promising micro-carrier for efficient colon targeting delivery tool with improved chemotherapeutic efficacy against colon cancer.
PAGES: 108-123  |  24 VIEWS  34 DOWNLOADS

How To Cite this Article:

Saumen Karan, Debjyoti Naskar, Souvik Debnath, Tapan Kumar Chatterjee. Evaluation of Anticancer Activity and In- Vitro Release Assay of a Polymeric Microsphere Formulation for Colon Targeted Delivery of 5-Fluorouracil using Biocompatible Natural Gum Katira.. 2019; 9(3): 108-123.