HPLC Estimation of L-DOPA in Phaseolus vulgaris and Cyamopsis tetragonoloba for the Anti-parkinson’s Activity

Author(s): Balvinder Kaur* and Jyoti Saxena

Abstract: Aim: The present study aims at finding the presence of Levodopa (L-DOPA) in pods of two widely recognized beans of Leguminosae family namely Phaseolus vulgaris and Cyamopsis tetragonoloba. L-DOPA (L- 3,4 – dihydroxyphenylalanine) is used as a gold standard to ease the symptoms of Parkinson’s disease. The disease is associated with gradual loss of nerve cells in the brain and decreased the ability to synthesize dopamine, causing motor symptoms. Phaseolus vulgaris also called common bean consumed enormously throughout the world and is the main source of dietary protein in America and African countries. Cyamopsis tetragonoloba (Guar) is a legume crop found in an arid region of India and Pakistan, India is the 90% contributor of total Guar bean production. Both the plants possess nutritive value due to the presence of a high amount of Amino acids and protein content. HPLC technique is applied for the quantitative estimation of L-DOPA present in pods of both the plants. Methods: RP-C18 column was used with a mobile phase composed of methanol: water (20: 80), isocratically eluted with a flow rate of 1mL/min. Sample quantity injected was 20 μL. The UV detection wavelength was 280 nm and retention time noted as 7.269±0.3min. Calibration curve obtained by injecting different concentration range of standard solution gives regression equation as Y= 10.154x + 1.4696 and correlation coefficient as 0.998. The amount of L-DOPA present in both the plants were measured with respect to standard levodopa. Result and Conclusion: The amount of levodopa present in Phaseolus vulgaris was found to be 0.566% and 0.174% in Cyamopsis tetragonoloba, which shows that both the plants contain L-DOPA in their pods. It can be concluded that RP-HPLC technique can be applied for quantitative estimation of levodopa present in plant extract and both the plants can be explored further to use in herbal drug formulations and can provide an alternative source of L-DOPA from a natural source to ease symptoms of Parkinson’s disease.

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