In Vitro Activities of Lawsonia inermis L. (Henna) Leaves Extract

Author(s): Ghodekar Anuradha S., Jarande Kajal S., Satav Pallavi S, Patil Neha and Waghmode Meghmala

Abstract: Lawsonia inermis (henna) is the medicinal plant. The study has been done to evaluate the preliminary phytochemical, proximate analysis and the bioactive potential of L. inermis. In phytochemical, qualitative test of flavonoid, phenol, steroids, resins, tannins and carbohydrates were found to be present. Thin layer chromatography was also done for the phytochemical analysis. Total phenol content by using Folin-Ciocalteau reagent and total flavonoid content were determine by using Woisky and Salatino method. Phenol and flavonoid content found to be 1158mg/g and 322.5mg/g respectively. In fluorescent analysis acetone, ethanol, methanol, glacial acetic acid, chloroform, diethyl ether, petroleum ether and benzene gave characteristics red fluorescence under long UV365 nm. Methanol extract of henna leaves found to have antimicrobial activity against Pseudomonas and Bacillus using disc and well diffusion method. Extract was used to evaluate antiarthritic potential. In which soxhlet water extract showed remarkable inhibition of protein denaturation and was found to be 71.41 ± 0.01. In thrombolytic activity aqueous extract was found to be significant which showed maximum about 52.62 ± 0.017 of clot lysis. The free radical scavenging by DPPH method of methanolic extract of henna leaves showed 71.7 ± 0.02 antioxidant activity. The soxhlet methanol extract of henna leaves showed 1101± 0.02 μg/ml antioxidant activity.

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