Qualitative and Quantitative Evaluation of Phytochemicals in Leaf Extract of Alstonia scholaris (L.) R. Br.By GC-MS Technique

Author(s): Mamta Goyal and *Laxmikant Shinde

Abstract: The aim of present research was to evaluate the various phytochemicals present in leaf extract of Alstonia scholaris (L.) R. Br. The essential oil was extracted by Soxhlet extraction method using methanol as solvent. To achieve qualitative and quantitative evaluation of secondary metabolites, extract was analyzed by gas chromatography and mass spectroscopy. GC-MS evaluation of methanolic leaf extract of Alstonia scholaris (L.) R. Br revealed the presence of 31 identified components. It constitutes of hydrocarbon moiety (42.44%), flavonoids (24.24%), tannins (9.7%) hydrocarbon derivatives (8.65%), alkaloid (3.61%), sesquiterpenes (2.98%), fatty acids (2.93%), phenols and alcohols (2.6%), sesquiterpenoid (1.36%), coumaran and ketones (1.73%), sugar moiety (0.62%), terpenoid (0.13%). These secondary metabolites (phytochemicals) provide evolutionary advantage to the plant in the form of protection against fungi, insects, microbes and environmental stress. The major components includes n-hexane (37.82%); alpha Methyl mannofuranoside (12.26%); 2-O-methyl-D-mannopyranosa (11.98%); quinic acid (9.70%); ethane,1- chloro-1-fluoro (8.47%); squalene (2.53%); 1,3-propanediol, 2-(hydroxymethyl)-2-nitro- (2.23%); 9,12-Octadecadienoic acid (Z, Z)- (2.13%); pentane, 2-methyl-(1.40%); pentane ,3-methyl-(1.38%); cyclopentane, methyl-(1.37%); and phytol (1.10%). The majority of phytochemicals in Alstonia scholaris found to possess numerous medicinal attributes viz. antifungal, antimalarial, antimicrobial, anti-fertility, antidiabetic, anthelmintic, antiparasitic activities. The plant also possesses broad spectrum of medicinal attributes against many ailments.

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