Bioactive Potential of Parthenium hysterophorus and Cytotoxicity Assay of Parthenin

Author(s): Pande Roshani A, Gole Akshata R, Sankpal Rohan V, Jadhav Pallavi B, Waghmode Meghmala S and Patil Neha N*

Abstract: Parthenium hysterophorus belongs to the family Asteriaceae. It is rich in hormones, carbohydrates, alkaloids, steroids, tannins, saponins etc. and hence can be used as traditional medicine. Solvent extracts of leaves was tested against pathogenic bacteria and fungi, by using Disk Diffusion method. Qualitative analysis of aqueous leaf extract was studied for the phytochemical screening. Phytochemical analysis shows the presence of steroids, phenol, saponins, tannin, flavonoids, terpanoids, carbohydrates etc. Fluorescence analysis of leaf powder was carried out. Parthenin compound was extracted from Parthenium hysterophorus and was tested for used for cytotoxicity activity using MBMD231, breast cancer cell line. Further studies were carried out on detection of anti-diabetic activity by two ways i.e. α-amylase inhibition which showed 75 ± 1.04 % activity and glucose diffusion inhibitory study showed 20% relative movement. Parthenium leaf extract have 74.3 ±0.3 % anti-oxidant activity using DPPH method while 0.81 ±0.04 mg of Trolox equivalent/ gram using FRAP assay. But only 18.18±0.02 % of anti-arthritic and 52.72 ± 0.018 % thrombolytic activity. Cytotoxic assay was on MDMB231 cell line showed that parthenin have very low cytotoxicity. Anti-helminthic activity was done using Asonia putida earthworm. No paralysis or death of earthworm was found within 24 hours by aqueous leaf extract of P. hysterophorus.

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