Characterization of Inulinase from Aspergillus niger AM270052.1 and Scale Up of Conditions for Mass Production

Author(s): V. Parivuguna, P. Saravana kumari and S. Shanmugham

Abstract: Microbial inulinase are of considerable interest both in fundamental studies and in industrial applications. The increasing potential of inulinase application prompts screening for newer inulinase producing microorganisms using cheap substrates which can meet the conditions favourable to the industrial applications. A soil isolate, Aspergillus niger AM270052.1 was confirmed as a potential candidate for the large-scale production of inulinase using agave tuber as a cheaper substrate. Using the isolate, the optimum pH and temperature for enzyme production was determined as 4.5 and 55oC. Inulin content in easily available agave tuber was comparatively equal to the inulin content available in the economically costlier substrate such as chicory, bulbs of onion and garlic. So, agave tubers were suggested in the study for the mass production of inulinase. The maximum yield of pure inulinase enzyme was obtained by DEAE-Sephadex ion-exchange chromatography purification technique from the fermented substrate under optimized conditions was 23.2%. Zymogram analysis showed the presence of single band with approximate molecular weight of 93 KDa. Further confirmation on inulinase activity affirmed by presence of glucose, fructose and sucrose from agave medium treated with enzyme by TLC technique. So, the study concludes that agave tuber as the cheaper substrates for production of inulinase for their applications in industrial purposes.

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