Development and Validation of Stability Indicating UPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Pharmaceutical Dosage Form

Author(s): Khansa*, Shyamala, J V C Sharma, V. Mohan Goud

Abstract: The objective of the method was to develop a simple, rapid, sensitive, precise, accurate and validated Ultra Performance Liquid Chromatographic (UPLC) method for the simultaneous estimation of Meropenem and Vaborbactam in pharmaceutical dosage form. Chromatographic separation was achieved on an acquity UPLC CHS C18 (2.1 x 100mm, 1.8μm) column with a mobile phase composed of 0.01N Potassium dihydrogen phosphate buffer and Acetonitrile in the ratio of 55:45 of 4.8pH at a flow rate of 0.2 ml/min and 1 μl injection volume. The effluents were detected at a wavelength of 260 nm using TUV detector. The retention times of Meropenem and Vaborbactam were found to be 0.665 and 0.875 min respectively. The method was validated with respect to specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Meropenem and Vaborbactam were found to be 0.999 and 0.999 respectively. Recovery of Meropenem and Vaborbactam in formulation was found to be 99.93% and 99.62% respectively. Due to simplicity, high precision and rapidness the method can be successfully applied for simultaneous estimation of Meropenem and Vaborbactam in combined dosage form.

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