Isolation, Identification and Characterization of Nitrate Reducing Bacteria from the Estuarine Waters of Selected Sampling Stations of Rajakkamangalam Estuary V.N.

Author(s): V.N. Hameetha Banu, P.Mohideen Askar Nawas*, H.F.Syed Mafiya haniff, Sujatha Ravi

Abstract: Nitrate reduction by bacteria is a crucial step in nitrogen cycle making the Nitrogen available for organisms. Nitrate reductase enzyme and the bacteria as a denitrifying organism find important in applications such as waste treatment, aquaculture pond restoration, to improve crop growth. Aimed at good nitrate reducers, bacteria were isolated from the estuarine waters of Rajakamangalam, Kanyakumari, Southwest Coast of India. A total of 19 dissimilar bacterial strains were isolated and screened for nitrate reducing activity. Four isolates showed significant nitrate reducing ability and are subsequently characterised phenotypically and genotypically. The 16S rRNA sequences of the isolates were deposited in the Genbank with their respective names as Marinobacter hydrocarbonoclasticus, Bacillus subtilis, Denitrovibrio acetiphilus, and Pseudomonas citronellolis.

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