Protein Precipitant Activity of Ocimum basilicum

Author(s): Venkatarao Vutla*, B. Kusuma Sowjanya, D. Tejaswini, E. Indumathi, J. Hari Sreekanth, T. Dileepkumar and Suryadevara Vidhyadhara

Abstract: Tannin fractions were isolated from Leaves of ocimumbasilicum, used tannin acid as standard. The total phenolic compound and were determined. It was found that the tannins from the leaves of tulasi had the highest total phenolic compound and the lowest hydrolysable tannins. The interaction between tannins fraction from plant sources and tannic acid (as standard) with casein precipitated from skim milk (by acidification) was determined using precipitating potential method. The optimum pH of the interactions was at pH 5, while the lowest interaction was at pH 7. The highest precipitating potential was noted for tannic acid followed by tulasi leaves. Moreover, the tannins-casein complex was determined using fluorescence quenching method. The interaction between casein and tannic acid had the most extensive fluorescence quenching for plant tannins.

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