Analysis of Phytochemical, Antioxidant and Anti-Inflammatory Activity of Cissus quadralangularis

Author(s): Kavya G, Akshatha M. D and Selvam Arjunan

Abstract: Cissus quadrangularis, a climber belonging to vitaceae family, is widely used in ayurvedic medical practices and believed to promote bone fracture healing. It is also used for the management of obesity, diabetics, asthma, menstrual discomfort, stomach ulcers, etc. Although traditional system of medicines values the plant for its profile and biological activity of plant for its medicinal properties, vigorous scientific analysis of the phytochemical studied are limited. This study looks at the phytochemical profile of the stem and node extract of C. quadrangularis and analyzed the antioxidant and anti-inflammatory of this extract to facilitate more controlled future studies to optimize a process for efficient in vitro propagation of the plant. Chloroform and Ethanol extracts from the plant were assayed qualitatively for secondary metabolites. The antioxidant activity of extracts was studied using DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) and electrochemical methods. Anti-inflammatory activity was analyzed using in vitro propagation method for standardizing C.quadrangularis by optimizing media composition and incubation conditions. The study showed that saponins, alkaloids, carbohydrates, cardiac glycosides and terpinoids are present in the plant extract of Cissus quadralangularis. Along with high concentration of phenolics, the extracts exhibited significant antioxidant and anti-inflammatory activity. Furthermore, media formulation that facilitated callus initiation from stem explants was developed.

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