Development and Validation of UV Spectrophotometric Methods for the Estimation of Propofol in Bulk and Pharmaceutical Formulations

Author(s): Anil Kumar S. M*, A. Satish Kumar Shetty and N. D. Satyanarayan

Abstract: Two simple and sensitive UV Spectrophotometric methods have been developed and validated for the estimation of propofol in bulk and pharmaceutical formulations. Method A is a zero order derivative method which exhibit absorption maximum at 272 nm by using methanol as the solvent and obeyed Beer’s law in the concentration range of 10 – 50 μg / ml. Method B is first order derivative method which shows good absorption at 282 nm and obeyed Beer’s law in the concentration range of 10 - 50 μg / ml. The results of analysis for both the methods have been validated statistically by recovery studies and the percentage relative standard deviation for both the methods were found to be below 2%. The proposed methods are sensitive, accurate, precise and economical for the routine estimation of propofol in bulk and pharmaceutical formulations.

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